Laboratory for biotechnology of physiological active substances

Head, Laboratory for Biotechnology of Physiologically Active Substances

Maryna M. Kornet

Associate Professor, Department of Chemistry ZNU

Phone/ messengers:  +38-067-802-56-31

E-mail: kornetmarina77@gmail.com, kornet.m@znu.edu.ua

CV Kornet M.

Website:

• https://www.researchgate.net/profile/Marina_Kornet
• https://www.scopus.com/authid/detail.uri?authorId=55860458800
• http://www.researcherid.com/rid/G-4711-2016
• https://www.linkedin.com/in/maryna-kornet-90b8453b/

The basic work direction is searching for new bioregulators among the natural and synthetic compounds.

The laboratory is open to cooperation and will gladly consider proposals for joint projects.

Examples of research:

1. Creating virtual combinatorial libraries and screening of potential biologically active substances.
2. Carrying out computer prediction of the biological effects of the compounds.
3. Synthesis and identification, determination of physicochemical constants azaheterocycles derivatives.
4. Testing of new materials at various levels (in vitro, in vivo, microorganisms, plants, animals).
5. Determination of correlations "structure-property-biological action."
6. The selection of the most promising substances for in-depth biological research, development of laboratory regulations, and effective dosage forms.

Biological researches:

1) The molecular level

• Antiradical activity

2) The cell level

• The cytoprotective activity

3) In isolated organs and cell groups

• Antioxidant activity (enzymatic and non-enzymatic initiation of free radical oxidation)
• Membrane stabilizing activity

4) On the intact organisms

• The antimicrobial activity
• Acute toxicity chemical
• Hepatoprotective activity
• Radioprotective activity
• Cytotoxic Activity
• The anti-inflammatory effect
• The analgesic effect

Some publications:

(1) Hodyna, D.; Kovalishyn, V.; Romanenko, Y.; Semenyuta, I.; Blagodatny, V.; Kachaeva, M.; Brazhko, O.; Metelytsia, L. Quinoline Hydrazone Derivatives as New Antibacterials against Multidrug Resistant Strains. Chemistry & Biodiversity 2023, 20 (9). DOI: 10.1002/cbdv.202300839.

(2) Holovatiuk, V. M.; Brazhko, O. A.; Kashkovsky, V. I. The acute toxicity and analgesic activity investigation of new spirocyclic pyrazolidine-3,5-dione derivatives. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University 2016, 6 (3), 149. DOI: 10.15421/201681.

(3) Kolomiiets, O. V.; Tsygankov, A. V.; Kornet, M. N.; Brazhko, A. A.; Musatov, V. I.; Chebanov, V. A. Synthesis of imidazo 1,2-a pyridine-containing peptido- mimetics by tandem of Groebke-Blackburn-Bienayme and Ugi reactions. Beilstein Journal of Organic Chemistry 2023, 19, 727-735. DOI: 10.3762/bjoc.19.53.

(4) Kornet, M. M.; Brazhko, O. A.; Zavhorodniy, M. P.; Tkach, V. V.; Kruglyak, O. S.; Ivanushko, Y. G.; de Oliveira, S. C.; Yagodynets, P. I. Electrochemical Determination of Antioxidant Activity of New 4-Thiosu bstituted Quinoline Derivatives with Potential Radioprotecting Properties. Biointerface Research in Applied Chemistry 2021, 11 (2), 9148-9156. DOI: 10.33263/briac112.91489156.

(5) Kornet, M. M.; Dudareva, G. F.; Gencheva, V. I.; Klimova, O. O.; Peretiatko, V. V.; Brazhko, O. A. Growth-regulatory Activity of 2-Methyl-4-thioquinoline Derivatives. Ukrainian Journal of Ecology 2020, 10 (2), 279-283. DOI: 10.15421/2020_97.

(6) Martynenko, Y. V.; Antypenko, O. M.; Brazhko, O. A.; Labenska, I. B.; Kovalenko, S. I. Directed Search of Biologically Active Compounds among Hydrogenated Isoindolylalkyl(alkylaryl-,aryl-)carboxylic Acids with Quinazoline Fragment that Modify the Carbohydrate Metabolism: Design, Synthesis and Modification. Acta Chimica Slovenica 2019, 66 (1), 145-154. DOI: 10.17344/acsi.2018.4731.

(7) Metelytsia, L.; Hodyna, D.; Dobrodub, I.; Semenyuta, I.; Zavhorodnii, M.; Blagodatny, V.; Kovalishyn, V.; Brazhko, O. Design of (quinolin-4-ylthio)carboxylic acids as new Escherichia coli DNA gyrase B inhibitors: machine learning studies, molecular docking, synthesis and biological testing. Computational Biology and Chemistry 2020, 85. DOI: 10.1016/j.compbiolchem.2020.107224.

(8) Murlykina, M. V.; Kolomiets, O. V.; Kornet, M. M.; Sakhno, Y. I.; Desenko, S. M.; Dyakonenko, V. V.; Shishkina, S. V.; Brazhko, O. A.; Musatov, V. I.; Tsygankov, A. V.; et al. Doebner-type pyrazolopyridine carboxylic acids in an Ugi four-component reaction. Beilstein Journal of Organic Chemistry 2019, 15, 1281-1288. DOI: 10.3762/bjoc.15.126.

(9) Murlykina, M. V.; Kornet, M. N.; Desenko, S. M.; Shishkina, S. V.; Shishkin, O. V.; Brazhko, A. A.; Musatov, V. I.; Van der Eycken, E. V.; Chebanov, V. A. New tricks of well-known aminoazoles in isocyanide-based multicomponent reactions and antibacterial activity of the compounds synthesized. Beilstein Journal of Organic Chemistry 2017, 13, 1050-1063. DOI: 10.3762/bjoc.13.104.

(10) Murlykina, M. V.; Sakhno, Y. I.; Desenko, S. M.; Konovalova, I. S.; Shishkin, O. V.; Sysoiev, D. A.; Kornet, M. N.; Chebanov, V. A. Features of switchable multicomponent heterocyclizations of salicylic aldehydes and 5-aminopyrazoles with pyruvic acids and antimicrobial activity of the reaction products. Tetrahedron 2013, 69 (44), 9261-9269. DOI: 10.1016/j.tet.2013.08.055.

(11) Murlykina, M. V.; Sakhno, Y. I.; Desenko, S. M.; Shishkina, S. V.; Shishkin, O. V.; Sysoiev, D. O.; Kornet, M. N.; Schols, D.; Goeman, J. L.; Van der Eycken, J.; et al. Study of the Chemoselectivity of Multicomponent Heterocyclizations Involving 3-Amino-1,2,4-triazole and Pyruvic Acids as Key Reagents, and Biological Activity of the Reaction Products. European Journal of Organic Chemistry 2015, 2015 (20), 4481-4492. DOI: 10.1002/ejoc.201500469.

(12) Murlykina, M.; Pavlovska, T.; Semenenko, O.; Kolomiets, O.; Sanin, E.; Morozova, A.; Kornet, M.; Musatov, V.; Kulyk, K.; Mazepa, A.; et al. Effective Three-Step Construction of Betulonic Acid Hybrids with Heterocycle-Containing Peptidomimetic Fragments. Chemistryselect 2023, 8 (27). DOI: 10.1002/slct.202301250.

(13) Shupeniuk, V. I.; Zavhorodnii, M. P.; Derevianko, N. P.; Taras, T. N.; Shkopynska, T. Y.; Brazhko, O. A.; Matkivskyi, M. P. Search of regulators among (7-chloroquinoline-4-ylthio)carbonic acids and triazoles of anthracendione for microclonal propagation of plants. Journal of Chemistry and Technologies 2023, 31 (1), 20-27. DOI: 10.15421/jchemtech.v31i1.271400.

(14) Tkach, V. V.; Kushnir, M. V.; de Oliveira, S. C.; Zavhorodnii, M. P.; Brazhko, O. A.; Kornet, M. M.; Luganska, O. V.; Kopiika, V. V.; Ivanushko, Y. G.; Mytchenok, M. P.; et al. The Theoretical Description for a Sucralose Electrochemical Cathodical Determination over a 9-9′-Diacridyl-modified Electrode. Orbital-the Electronic Journal of Chemistry 2021, 13 (3), 219-222. DOI: 10.17807/orbital.v13i3.1584.

(15) Yakovleva-Nosar, S. O.; Derevyanko, N. P.; Yevlash, A. S.; Brazhko, O. A.; Zavhorodnii, M. P.; Tkach, V. V.; Yagodynets, P. I. A Search of the Efficient S-Hetarylsuccinate Landscape Design Plant Growth Stimulators. Biointerface Research in Applied Chemistry 2022, 12 (1), 465-469. DOI: 10.33263/briac121.465469.

(16) Zavhorodnii, M. P.; Derevianko, N. P.; Shkopynska, T. Y.; Kornet, M. M.; Brazhko, O. A. Influence of 3-((6-R-quinolin-4-yl)thio)propanoic acid derivatives on rhizogenesis of pink rose clones (Rosa Damascena Mill.). Journal of Chemistry and Technologies 2022, 30 (4), 502-512. DOI: 10.15421/jchemtech.v30i4.265167.

(17) Zavhorodnii, M.; Derevianko, N.; Shkopynska, T.; Kornet, M.; Brazhko, O. Influence of derivatives of 2-((6-r-quinolin-4-yl)thio)acetic acid on rhizogenesis of Paulownia clones. Regulatory Mechanisms in Biosystems 2022, 13 (3), 213-218. DOI: 10.15421/022227.

(18) Shvets, V.; Maslak, H.; Davydov, V.; Berest, H.; Nosulenko, I.; Voskoboinik, O.; Omelianchyk, L.; Brazhko, O. Effects of the Aronia Melanocarpa extract action on the activity of mitochondrial creatine kinase under immobilization stress in old rats. Hacettepe University Journal of the Faculty of Pharmacy 2023, 43(4), 333-339. DOI: https://doi.org/10.52794/hujpharm.1269999

(19) Kovaleva, A.; Osmachko, A.; Ilina Т.; Goryacha, O.; Omelyanchik, L.; Grytsyk, A.; Koshovyi, O. Chemical composition of essential oils from flowers of veronica Longifolia L., Veronica Incana L. and Veronica Spicata L. ScienceRise: Pharmaceutical Science 2022, 4 (38), 69–79. DOI: https://doi.org/10.15587/2519-4852.2022.263735

Some monographs:

1. Modern Aspects Of Drugs Creation Based On QuS-Program Development. Oleksandr Brazhko, Viktoriya Gencheva, Maryna Kornet, Mikhailo Zavhorodniy. LAP LAMBERT Academic Publishing. 2020. 72 p. ISBN-10: 6202923199
2. S-хінолілзаміщені амінотіолів та тіокислот: синтез, фізико-хімічні властивості та біологічна активність : монографія. M. Корнет, A. Амінова, O. Бражко, M. Завгородній. Запоріжжя : Видавничий дім «Гельветика», 2021. 220 с. ISBN 978-966-992-606-7).
3. S,N-modifiedaminothiols as antioxidants and potential anti-radiation agents : monograph / M. Kornet, O. Brazhko, M. Zavgorodnii, N. Uzlenkova, A. Aminova. Riga, Latvia : “Baltija Publishing”, 2021. 204 p. ISBN 978-9934-26-124-4

Some patents:

1. Patent 93850 Ukraine, IPC C07D 401/12 (2006.01). N,S-bis(2-methylquinolin-4-yl)cysteamines and their salts. O.A. Brazhko, M.M. Kornet, M.P. Zavhorodnii, O.O. Brazhko. No. a 2010 13724; declared on November 19, 2010; published on March 10, 2011. Bulletin No. 5. 5 p.
2. Patent 97937 Ukraine, IPC C07D 215/36, C07D 219/04, C07D 221/06, C07D 221/16, C07D 319/14. S-(azaheteryl)cysteamines and their salts. Brazhko O.A., Kornet M.M., Zavhorodniy M.P. No. a 2011 11474; declared on 28.09.2011; published on March 26, 2012. Bulletin No. 6. 10 p.
3. Utility model patent 88165 Ukraine, IPC (51) А61К 47/18 (2006.01), А61К 47/22 (2006.01). The method of radiation protection against acute radiation injuries. Uzlenkova N.E., Nenyukova O.V., Kornet M.M., Brazhko O.A. No. u 2013 06184; declared on 20.05.2013; published on 11.03.2014. Bulletin No. 5. 4 p.
4. Utility model patent 152250 Ukraine, (51) IPC (2022.01), C07B 39/00, C07B 41/08, (2006.01), C07B 45/06 (2006.01), C07D 215/00. Synthesis method of α-chloro-substituted β-(2-methyl-6-r-quinolin-4-ylthio)propionic acids. O. A. Brazhko, M. P. Zavhorodniy, M. M. Kornet, O. O. Klimova No. u 2021 07521; declared on 22.12.2021; published on 11.01.2023. Bulletin No. 2. 7 p.
5. Utility model patent 152204 Ukraine, (51) IPC (2022.01), C07B 41/02, C07B 41/08, (2006.01), C07B 45/06 (2006.01), C07D 215/00. Method of synthesis of α-hydroxy substituted β-(2-methyl-6-r-quinolin-4-ylthio)propionic acids. O. A. Brazhko, M. P. Zavhorodniy, M. M. Kornet, O. O. Klimova No. u 2021 07522; declared on 22.12.2021; published on 04.01.2023. Bulletin No. 1. 7 p.

Laboratory projects:

1. Title: Targeting advanced aminothiol-based radioprotective agents derivatized by quinolines. Term: 2023 – 2025. Funding: MSCA4Ukraine consortium and Alexander von Humboldt Foundation, University Heinrich Heine, Düsseldorf, Germany.
2. Title: Synthesis of S,N-modified aminothiols as potential anti-radiation agents. Term: 2022. Funding: DAAD, Research Stays for University Academics and Scientists, 2021.
3. Title: Rational design of S,N-modified aminothiols as potential anti-radiation agents. Term: 2019 – 2021. Funding: Ministry of Education and Science of Ukraine.
4. Title: Open Air Lecture Hall. Term: 2017 – 2018. Funding: British Council in Ukraine.
5. Title: Creation of biologically active substances based on S-substituted endogenous sulfur-containing compounds, state registration № 0115U000763. Term: 2015 – 2017. Funding: Ministry of Education and Science of Ukraine.
6. Title: Investigation of the biological activity of S- and N-substituted six-membered nitrogen-containing heterocycles, state registration № 0112U003062. Term: 2012 – 2014. Funding: Ministry of Education and Science of Ukraine.
7. Title: The synthesis of biologically active substances based on N- and S-substituted sulfur-containing amino acids and their analogs, state registration № 0106U000585. Term: 2009 – 2011. Funding: Ministry of Education and Science of Ukraine.
8. Title: Search for biologically active substances based on thio- and hydrazine-derivatives of azaheterocycles, state registration № 0104U010164. Term: 2006 – 2009. Funding: Ministry of Education and Science of Ukraine.